The social amoeba Dictyostelium discoideum undergoes a developmental transition into a multicellular organism when starved. The evolutionary conserved protein coronin A plays a crucial role in the initiation of development. Using aggregation assays as our main method, we aim to elucidate the role of coronin A in early development.
Drexler, S. K., Brogna, F., Vinet, A., Pieters, J. Investigating the Function of Coronin A in the Early Starvation Response of Dictyostelium discoideum by Aggregation Assays. J. Vis. Exp. (112), e53972, doi:10.3791/53972 (2016).