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Journal / Biology / Genome Redigering i
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Biology

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JoVE Journal Biology
Genome Redigering i
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
Journal (Biology)
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay
Journal (Biology)
Demonstration of Heterologous Complexes formed by Golgi-Resident Type III Membrane Proteins using Split Luciferase Complementation Assay
 
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Genome Redigering i

Article DOI: 10.3791/54113-v • 07:42 min • June 20th, 2016
June 20th, 2016 •
1, 2, 3
1Genetics, Development and Cell Biology, Iowa State University, 2Department of Biological Sciences, University of Cincinnati, 3Department of Biology, University of Maryland

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Gene-targeting mutagenese er nu muligt i en bred vifte af organismer ved anvendelse af genom redigeringsteknikker. Her demonstrerer vi en protokol for målrettet gen mutagenese hjælp transskription aktivator som effektor nukleaser (Talens) i Astyanax mexicanus, en fiskeart, der omfatter overflade fisk og cavefish.

Tags

Molekylærbiologi , Cavefish Talens Talen genom-redigering
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