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Competitive Transplants to Evaluate Hematopoietic Stem Cell Fitness

Article DOI: 10.3791/54345-v • 08:53 min • August 31st, 2016
August 31st, 2016 •
1, 1
1INRS-Institut Armand-Frappier, Université du Québec, Institut National de la Recherche Scientifique

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Summary
Automatic Translation

This protocol provides step-by-step guidelines for setting up competitive mouse bone marrow transplant experiments to study hematopoietic stem/progenitor cell function without prior purification of stem cells by cell sorting.

Tags

Competitive Transplants Hematopoietic Stem Cells Fitness Evaluation In Vivo Transplant Assay Stem Cell Biology Hematopoietic Cell Transplantation Manipulations Self-renewal Limited Number Of Animals Cell Sorting Immunology Mixed Bone Marrow Chimeras Development Of Technique Roxanne Hetu-Arbour Edward Owusu Kwarteng Surgical Platform Skin And Fur Wetting Hind Foot Cutting Excess Muscle Removal
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