Eine schnelle und effiziente Erzeugung von rekombinanten humanen pluripotenten Stammzellen durch Rekombinase-vermittelten Kassettenaustausch in der<em> AAVS1</em> Locus
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November 20, 2016
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Hier berichten wir eine schnelle und effiziente Gen Editierverfahren basierend auf RMCE im AAVS1 Locus der menschlichen pluripotenten Stammzellen (hPSCs), die auf die zuvor beschriebenen Systeme verbessert. Mit dieser Technik können isogene Linien schnell und zuverlässig für die richtige Vergleichsstudien erzeugt, erleichtert Transgenität vermittelte Forschung mit hPSCs.
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Cite this Article
Ordovás, L., Boon, R., Pistoni, M., Chen, Y., Sambathkumar, R., Helsen, N., Vanhove, J., Berckmans, P., Cai, Q., Vanuytsel, K., Raitano, S., Verfaillie, C. M. Rapid and Efficient Generation of Recombinant Human Pluripotent Stem Cells by Recombinase-mediated Cassette Exchange in the AAVS1 Locus. J. Vis. Exp. (117), e54718, doi:10.3791/54718 (2016).
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