Bestimmung der relativen Zelloberfläche und Total Expression rekombinanter Ionenkanäle Mit Flow Cytometry
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11:32 min
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September 28, 2016
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Summary
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Ererbten Arrhythmien werden häufig durch Mutationen verursacht, die die Oberfläche Lieferung eines oder mehrerer Ionenkanäle verändern. Hier passen wir die Durchflusszytometrie-Assays eine Quantifizierung der relativen Gesamt und Zelloberflächenproteinexpression von rekombinanten Ionenkanäle in tsa-201-Zellen exprimiert zu liefern.
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Cite this Article
Bourdin, B., Segura, E., Tétreault, M., Lesage, S., Parent, L. Determination of the Relative Cell Surface and Total Expression of Recombinant Ion Channels Using Flow Cytometry. J. Vis. Exp. (115), e54732, doi:10.3791/54732 (2016).
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