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Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy
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JoVE Journal Chemie
Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

Insights into the Interactions of Amino Acids and Peptides with Inorganic Materials Using Single-Molecule Force Spectroscopy

DOI:
10.3791/54975-v

•

05:44 min

•

March 06, 2017

•

, ,
1Institute of Chemistry and The Center for Nanoscience and Nanotechnology,The Hebrew University of Jerusalem

Kapitel

  • 00:05Titel
  • 00:40AFM Tip Modification
  • 03:38Single-molecule Force Spectroscopy Measurements
  • 04:41Results: Measuring Single Molecule Adhesion Events
  • 05:18Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here we present a protocol to measure the force of interactions between a well-defined inorganic surface and either peptides or amino acids by single-molecule force spectroscopy measurements using an atomic force microscope (AFM). The information obtained from the measurement is important to better understand the peptide-inorganic material interphase.

Tags

Single-molecule Force SpectroscopyAmino AcidsPeptidesInorganic MaterialsSurface ChemistryCompositesChordatesAFM CantileversSilane CompoundsFmoc-PEG-NHSPiperidineN-Methyl-2-pyrrolidoneAmino Acid Coupling

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