In vitro Differenzierung von humanen CD4 + FOXP3 + Induced regulatorischer T - Zellen (iTregs) von Naïve CD4 + T - Zellen unter Verwendung eines TGF-β-enthaltende Protokoll
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December 30, 2016
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Dieses Protokoll beschreibt die reproduzierbare Erzeugung und Phänotypisierung von humanen regulatorischen T - Zellen induziert (iTregs) von naiven CD4 + T - Zellen in vitro. Verschiedene Protokolle für FOXP3 Induktions ermöglichen die Untersuchung spezifischer iTreg Phänotypen mit jeweiligen Protokollen erhalten.
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Schmidt, A., Éliás, S., Joshi, R. N., Tegnér, J. In Vitro Differentiation of Human CD4+FOXP3+ Induced Regulatory T Cells (iTregs) from Naïve CD4+ T Cells Using a TGF-β-containing Protocol. J. Vis. Exp. (118), e55015, doi:10.3791/55015 (2016).
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