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Isolation of Intact Mitochondria von Skeletal Muscle durch differentielle Zentrifugation für hochauflösende Messungen Respirationstest
Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements
JoVE Journal
Biologie
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JoVE Journal Biologie
Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements

Isolation of Intact Mitochondria von Skeletal Muscle durch differentielle Zentrifugation für hochauflösende Messungen Respirationstest

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10:25 min

March 08, 2017

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10:25 min
March 08, 2017

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Summary

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Here, a quadriceps muscle specimen is taken from an anaesthetized pig and mitochondria are isolated by differential centrifugation. Then, the respiratory rates of mitochondrial respiratory chain complexes I, II and IV are determined using high-resolution respirometry.

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