Genome Editing und gerichteten Differenzierung von hPSCs zur Abfrage Lineage Bestimmungsfaktoren im Bereich Human Pancreatic Entwicklung
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March 05, 2017
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Summary
Automatically generated
Protokolle HPSC Mutantenlinien mit der iCRISPR Plattform und zu differenzieren hPSCs in Glukose-responsive β-ähnlichen Zellen beschrieben zu erzeugen. Genom Bearbeitungstechnologie mit HPSC gerichtete Differenzierung Die Kombination bietet eine leistungsfähige Plattform für die systematische Analyse der Rolle von Lineage Determinanten in der menschlichen Entwicklung und Progression der Erkrankung.
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Cite this Article
Shi, Z., Soh, C., Zhu, Z., Huangfu, D. Genome Editing and Directed Differentiation of hPSCs for Interrogating Lineage Determinants in Human Pancreatic Development. J. Vis. Exp. (121), e55267, doi:10.3791/55267 (2017).
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