Ein HS-MRM-Assay für die Quantifizierung der Wirtszelle Proteine in Protein Biopharmazeutika durch flüssige Chromatographie Ion Mobility QTOF Massenspektrometrie
10,083 Views
•
11:09 min
•
April 17, 2018
DOI:
Summary
Automatically generated
Hier beschreiben wir einen chromatographischen Assay, gekoppelt mit der Ionen-Mobilität Trennung von Peptid-Vorstufen, gefolgt von den hochauflösenden (~ 30.000) MS-Detektion von Peptid-Fragmente für die Quantifizierung der Spike Peptid Standards in ein monoklonaler Antikörper Digest.
Explore More Videos
Verwandte Videos
10:37
Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Verwandte Videos
11926 Views
08:53
Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
Verwandte Videos
9789 Views
N/A
Carrier-assisted One-pot Sample Preparation for Targeted Proteomics Analysis of Small Numbers of Human Cells
Verwandte Videos
2125 Views
09:40
A Mass Spectrometry-Based Proteomics Approach for Global and High-Confidence Protein R-Methylation Analysis
Verwandte Videos
2315 Views
05:52
Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry
Verwandte Videos
1073 Views
09:45
Host Cell Protein Analysis using Enrichment Beads Coupled with Limited Digestion
Verwandte Videos
2201 Views
11:09
An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry
Verwandte Videos
10.1K Views
05:52
Histone Modification Screening using Liquid Chromatography, Trapped Ion Mobility Spectrometry, and Time-Of-Flight Mass Spectrometry
Verwandte Videos
1.1K Views
08:56
Detection of Regulated Ergot Alkaloids in Food Matrices by Liquid Chromatography-Trapped Ion Mobility Spectrometry-Time-of-Flight Mass Spectrometry
Verwandte Videos
603 Views
08:06
Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry
Verwandte Videos
9.2K Views
Read Article
Cite this Article
Doneanu, C., Fang, J., Alelyunas, Y., Yu, Y. Q., Wrona, M., Chen, W. An HS-MRM Assay for the Quantification of Host-cell Proteins in Protein Biopharmaceuticals by Liquid Chromatography Ion Mobility QTOF Mass Spectrometry. J. Vis. Exp. (134), e55325, doi:10.3791/55325 (2018).
Copy