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Preparation of Non-human Primate Brain Tissue for Pre-embedding Immunohistochemistry and Electron Microscopy

Article DOI: 10.3791/55397-v • 11:55 min • April 3rd, 2017
April 3rd, 2017 •
1,2, 1
1Centre de recherche de l'Institut universitaire en santé mentale de Québec, Department of Psychiatry and Neuroscience, Université Laval, 2Centre de recherche du CHU Sainte-Justine

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Summary
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Here, we provide an easy, low-cost, and time-efficient protocol to chemically fix primate brain tissue with acrolein fixative, allowing for long-term preservation that is compatible with pre-embedding immunohistochemistry for transmission electron microscopy.

Tags

Pre-embedding Immunohistochemistry Electron Microscopy Non-human Primate Brain Tissue Synaptic Incidence Neuroanatomy Labeling Innervations Acrolein Fixation Sodium Borohydride Solution PBS Wash Blocking Solution Normal Serum Fish Gelatin Primary Antibody Solution
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