Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Seyyed Hamidreza Hashemipetroudi; Ghorbanali Nematzadeh; Gholamreza Ahmadian; Ahad Yamchi; Markus Kuhlmann

doi:10.3791/55451

Journal

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Genetik

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Bewertung der Kontamination der DNA in RNA-Proben anhand der ribosomalen DNA

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

JoVE Journal
Genetik

This content is Free Access.

JoVE Journal Genetik

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Bewertung der Kontamination der DNA in RNA-Proben anhand der ribosomalen DNA

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21,273 Views

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13:16 min

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January 22, 2018

DOI:

10.3791/55451-v

DOI:

10.3791/55451-v

•

13:16 min

January 22, 2018

•

18 Views

Seyyed Hamidreza Hashemipetroudi¹, Ghorbanali Nematzadeh¹, Gholamreza Ahmadian², Ahad Yamchi³, Markus Kuhlmann⁴

¹Genetic and Agricultural Biotechnology Institute of Tabarestan (GABIT),Sari Agricultural Sciences and Natural Resources University (SANRU), ²Department of Industrial and Environmental Biotechnology,National Institute of Genetics Engineering and Biotechnology (NIGEB), ³Department of Plant Breeding and Biotechnology,Gorgan University of Agricultural Sciences & Natural Resources, ⁴RG Abiotic Stress Genomics/ RG Heterosis, Department Molecular Genetics,Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)

Transkript

Summary

Hier präsentieren wir Ihnen ein Protokoll zur Rückverfolgung genomische DNA (gDNA) Kontamination in RNA-Proben. Die vorgestellte Methode nutzt spezifische Primer für die internen übertragenen Distanzscheibe Region (ITS) der ribosomalen DNA (rDNA) Gene. Die Methode eignet sich für zuverlässige und empfindlichen Nachweis von DNA-Kontaminationen in den meisten Eukaryoten und Prokaryoten.