Battery of Behavioral Tests Assessing General Locomotion, Muscular Strength, and Coordination in Mice

Karlaina JL Osmon; Meera Vyas; Evan Woodley; Patrick Thompson; Jagdeep S Walia

doi:10.3791/55491

Batterie des Verhaltens testet Beurteilung der allgemeinen Fortbewegung, Muskelkraft und Koordina...

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JoVE Journal Behavior

Battery of Behavioral Tests Assessing General Locomotion, Muscular Strength, and Coordination in Mice

Batterie des Verhaltens testet Beurteilung der allgemeinen Fortbewegung, Muskelkraft und Koordination bei Mäusen

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06:54 min

January 23, 2018

DOI: 10.3791/55491-v

Karlaina JL Osmon¹, Meera Vyas¹, Evan Woodley², Patrick Thompson², Jagdeep S Walia^1,2,3

¹Centre for Neuroscience Studies,Queen's University, ²Department of Biomedical and Molecular Sciences,Queen's University, ³Medical Genetics/ Department of Pediatrics, Kingston General Hospital, Centre for Neuroscience Studies, and Department of Biomedical and Molecular Sciences,Queen's University

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Overview

This article discusses a battery of behavioral tests designed to assess general locomotion, muscular strength, and coordination in mice. These tests are crucial for evaluating the effects of treatments in preclinical trials.

Key Study Components

Area of Science

Neuroscience
Behavioral Testing
Preclinical Trials

Background

Behavioral testing is essential for assessing animal wellbeing.
It helps in understanding the phenotypic effects of diseases and treatments.
Multiple dimensions of movement are evaluated through various tests.
Common tests include the open field test, mesh test, and rotarod test.

Purpose of Study

To observe locomotion, strength, and coordination in mice.
To evaluate treatment outcomes on motility.
To provide a comprehensive assessment of motor functioning.

Methods Used

Open field test for general locomotion.
Mesh test for muscular strength.
Rotarod test for coordination.
Use of SmartWare software for data collection.

Main Results

Behavioral tests provide insights into treatment efficacy.
Multiple facets of movement are measured.
Results can inform future preclinical trials.

Conclusions

Behavioral testing is a valuable tool in neuroscience research.
It aids in understanding the impact of treatments on animal models.
Comprehensive assessments can lead to better therapeutic strategies.

Frequently Asked Questions

What is the purpose of behavioral testing in preclinical trials?

Behavioral testing assesses the phenotypic effects of diseases or treatments on animal wellbeing.

What tests are included in the behavioral assessment?

The assessment includes the open field test, mesh test, and rotarod test.

How does the open field test work?

The open field test measures general locomotion by observing the movement of mice in an open arena.

What does the mesh test evaluate?

The mesh test evaluates muscular strength by assessing the ability of mice to traverse a mesh surface.

What is the significance of the rotarod test?

The rotarod test measures coordination by evaluating how long mice can stay on a rotating rod.

Verhaltens testen in vorklinischen Studien dient um die phänotypischen Auswirkungen einer Krankheit oder Behandlung auf das Wohlbefinden des Tieres zu beurteilen. Bei der Beurteilung Global motor funktionieren, wir Tests für allgemeine Fortbewegung, Muskelkraft und Koordination ausgewählt: das freie Feld zu testen, das Netz und die Rotarod Test bzw..

Das übergeordnete Ziel dieser Reihe von Verhaltenstests ist es, die allgemeine Fortbewegung, Muskelkraft und Koordination bei Mäusen zu beobachten. Diese Methoden können dazu beitragen, wichtige Fragen zur Implementierung verschiedener Behandlungen in mehreren präklinischen Studien zu beantworten, z. B. zum Nutzen der Behandlungsergebnisse für die Motilität bei Mäusen. Der Hauptvorteil dieser Technik besteht darin, dass mehrere Facetten und Dimensionen der Bewegung durch diese Batterie von Verhaltenstests gemessen und bewertet werden.

Das Verfahren wird von Meera, einer Masterstudentin in unserem Labor, demonstriert. Schalten Sie zunächst das Open-Field-Testprogramm ein und verbinden Sie den Computer mit der Kamera oder der Open-Field-Box. Starten Sie die selektive SmartWare-Software über das Open Field Icon.

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