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Assaying Protein Kinase Activity with Radiolabeled ATP

Assaying Protein Kinase Activity with Radiolabeled ATP

Article DOI: 10.3791/55504-v 08:05 min May 26th, 2017
May 26th, 2017



Protein kinases are highly evolved signaling enzymes and scaffolds that are critical for inter- and intracellular signal transduction. We present a protocol for measuring kinase activity through the use of radiolabeled adenosine triphosphate ([γ-32P] ATP), a reliable method to aid in elucidation of cellular signaling regulation.


Keywords: Protein Kinase Activity Radiolabeled ATP Kinase Signaling Kinase Assay Cell Lysates Protein A-Sepharose Beads Kinase Reaction Buffer SDS-PAGE Coomassie Stain Autoradiography
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