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High Precision FRET at Single-molecule Level for Biomolecule Structure Determination
JoVE Journal
Biochemie
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JoVE Journal Biochemie
High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

High Precision FRET at Single-molecule Level for Biomolecule Structure Determination

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11:24 min

May 13, 2017

DOI:

11:24 min
May 13, 2017

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Transkript

Automatically generated

The overall goal of this protocol is to perform high precision inter-dye distance measurements using multi-parameter fluorescence detection of F

Summary

Automatically generated

A protocol for high-precision FRET experiments at the single molecule level is presented here. Additionally, this methodology can be used to identify three conformational states in the ligand-binding domain of the N-methyl-D-aspartate (NMDA) receptor. Determining precise distances is the first step towards building structural models based on FRET experiments.

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