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Neonatal Murine Cochlear Explant Technique as an In Vitro Screening Tool in Hearing Research

Article DOI: 10.3791/55704-v • 08:30 min • June 8th, 2017
June 8th, 2017 •
1,2,3, 1,4, 1,2,4
1Eaton Peabody Laboratories, Department of Otolaryngology, Massachusetts Eye and Ear, 2Department of Otolaryngology, Harvard Medical School, 3Department of Otolaryngology, Vienna General Hospital, Medical University of Vienna, 4Harvard Program in Speech and Hearing Bioscience and Technology

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Summary
Automatic Translation

The goal of this protocol is to demonstrate the preparation, culture, treatment, and immunostaining of neonatal murine cochlear explants. The technique can be utilized as an in vitro screening tool in hearing research.

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Neonatal Murine Cochlear Explant Technique In Vitro Screening Tool Hearing Research Culture Treatment Immunostaining Cochlear Hair Cells Synapses Neurites Neurons Ex Vivo Model Mechanisms Of Action Candidate Therapeutic Compounds Testing In Vivo Neonate Head Scalpel Blade External Auditory Canals Cranium Sagittal Suture Compression Of The Cochlea Snout Brain And Cochlea Forceps
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