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JoVE Journal
Developmental Biology

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Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development
 

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Article DOI: 10.3791/55823-v 09:32 min June 15th, 2017
June 15th, 2017

Kapitel

Summary

This protocol describes an optogenetic strategy to modulate mitogen-activated protein kinase (MAPK) activity during cell differentiation and Xenopus embryonic development. This method allows for the reversible activation of the MAPK signaling pathway in mammalian cell culture and in multicellular live organisms, like Xenopus embryos, with high spatial and temporal resolution.

Tags

Keywords: Light-mediated Reversible Modulation Mitogen-activated Protein Kinase Pathway Cell Differentiation Xenopus Embryonic Development Optogenetic Induction CIBN-CRY2 Interaction Fluorescence Imaging PHK21 Cells CRY2-mCherry-Raf1 Plasmid
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