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A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
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A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells

Article DOI: 10.3791/55841-v • 09:05 min • August 7th, 2017
August 7th, 2017 •
1, 1, 1, 1, 1, 1
1Department of Internal Medicine, Leiden University Medical Centre

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Summary
Automatic Translation

Here we present a novel clinical grade isolation and culture method for kidney Perivascular Stromal Cells (kPSCs) based on whole organ perfusion with digestive enzymes and NG2-cell enrichment. With this method, it is possible to acquire sufficient cell numbers for cellular therapy.

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Clinical Grade Isolation Method Human Kidney Perivascular Stromal Cells KPSC Cell Therapeutic Treatment Regenerative Medicine Kidney Diseases Transplantation Functional Testing Perfusion Tray Water Bath L/S 25 Tube Sterile Gauze Medium Pump Speed
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