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In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption

Article DOI: 10.3791/55846-v • 10:36 min • June 20th, 2017
June 20th, 2017 •
1, 1, 1, 1
1Department of Anatomy and Cell Biology, University of Illinois at Chicago

Kapitel

Summary
Automatic Translation

Maintaining blood-brain barrier coverage is key for the homeostasis of the central nervous system. This protocol describes in vitro techniques to delineate the fundamental and pathological processes that modulate blood-brain barrier coverage.

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In Vitro Assays Blood-brain Barrier Mesh-like Vessel Formation Disruption Disease-relevant Processes Underlying Mechanisms Potential Treatments In Vitro Testing Basic Research Questions Applied Research Questions Neuro-degeneration Fields Disease-relevant Stresses Preformed Network Structures Underlying Pathways Stress-induced Blood-brain Barrier Disruption In Vivo Testing Alzheimer's Disease Homeostatic Functions Cognition Blood-brain Barrier Dysfunction Neurodegenerative Disorders Stroke HCMEC/D3 Immortal Endothelial Cells Calcium And Magnesium-free HBSS 0.25% Trypsin EDTA EBM-2 Complete Medium
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