Phasenverhalten von geladenen Vesikeln unter symmetrischen und asymmetrischen Lösungsbedingungen überwacht mit Fluoreszenz-Mikroskopie
9,192 Views
•
10:08 min
•
October 24, 2017
DOI:
Summary
Automatically generated
Experimente an Phase getrennt giant Unilamellar Vesikeln (GUVs) vernachlässigen häufig physiologische Lösungsbedingungen. Dieses Werk stellt Ansätze, um die Auswirkungen der hohen Salzgehalt Puffer auf flüssig / flüssig Phasentrennung in aufgeladenen Mehrkomponenten GUVs als Funktion des Trans-Membran Lösung Asymmetrie und Temperatur zu studieren.
Verwandte Videos
08:46
Studying Synaptic Vesicle Pools using Photoconversion of Styryl Dyes
Verwandte Videos
11709 Views
12:15
Single Liposome Measurements for the Study of Proton-Pumping Membrane Enzymes Using Electrochemistry and Fluorescent Microscopy
Verwandte Videos
7433 Views
08:15
Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients
Verwandte Videos
7954 Views
20:00
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
Verwandte Videos
13921 Views
07:33
Fluorescent Leakage Assay to Investigate Membrane Destabilization by Cell-Penetrating Peptide
Verwandte Videos
6297 Views
08:26
Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
Verwandte Videos
2401 Views
13:30
Mass-Sensitive Particle Tracking to Characterize Membrane-Associated Macromolecule Dynamics
Verwandte Videos
4025 Views
13:47
TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
Verwandte Videos
10805 Views
10:58
SNARE-mediated Fusion of Single Proteoliposomes with Tethered Supported Bilayers in a Microfluidic Flow Cell Monitored by Polarized TIRF Microscopy
Verwandte Videos
10860 Views
09:03
Giant Liposome Preparation for Imaging and Patch-Clamp Electrophysiology
Verwandte Videos
22213 Views
Read Article
Cite this Article
Kubsch, B., Robinson, T., Steinkühler, J., Dimova, R. Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy. J. Vis. Exp. (128), e56034, doi:10.3791/56034 (2017).
Copy