Journal
/
Neurowissenschaften
/
Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
JoVE Journal
Neurowissenschaften
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Neurowissenschaften
Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

DOI:
10.3791/56063-v

•

10:12 min

•

August 09, 2017

•

, , , ,
1Brain Institute,Federal University of Rio Grande do Norte, 2Department of Biosystems Science and Engineering,ETH Zurich, 3The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University

Kapitel

  • 00:05Titel
  • 00:57Dorsolateral Telencephalon Microdissection and Cell Plating
  • 04:23Time-lapse Video Microscopy
  • 05:24Cell Tracking
  • 08:12Results: Cell Lineage of Single Progenitor Cells
  • 09:41Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Live imaging is a powerful tool to study cellular behaviors in real time. Here, we describe a protocol for time-lapse video-microscopy of primary cerebral cortex cells that allows a detailed examination of the phases enacted during the lineage progression from primary neural stem cells to differentiated neurons and glia.

Tags

Live ImagingPrimary Cerebral Cortex Cells2D Culture SystemNeural Stem CellsNeuronsGlial CellsNeurodevelopmentCell LineageCell DivisionCell CycleCell GrowthEmbryonic BrainDorsolateral TelencephalonCell DissociationTrypsin-EDTAProliferation MediumPoly-D-lysine

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Magnetic Resonance Spectroscopy of live Drosophila melanogaster using Magic Angle Spinning

Chronic Imaging of Mouse Visual Cortex Using a Thinned-skull Preparation

Live-imaging of PKC Translocation in Sf9 Cells and in Aplysia Sensory Neurons

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae

Detection of Microregional Hypoxia in Mouse Cerebral Cortex by Two-photon Imaging of Endogenous NADH Fluorescence

Visualization and Genetic Manipulation of Dendrites and Spines in the Mouse Cerebral Cortex and Hippocampus using In utero Electroporation

Simultaneous Electroencephalography, Real-time Measurement of Lactate Concentration and Optogenetic Manipulation of Neuronal Activity in the Rodent Cerebral Cortex

Functional Imaging of Auditory Cortex in Adult Cats using High-field fMRI

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Live Imaging of Drosophila Larval Neuroblasts

Read Article