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Immunology and Infection

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JoVE Journal Immunology and Infection
In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues
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In Situ MHC-tetramer Staining and Quantitative Analysis to Determine the Location, Abundance, and Phenotype of Antigen-specific CD8 T Cells in Tissues

Article DOI: 10.3791/56130-v • 08:37 min • September 22nd, 2017
September 22nd, 2017 •
1, 1, 1
1Department of Veterinary and Biomedical Sciences, University of Minnesota

Kapitel

Summary
Automatic Translation

Here, we describe a method that combines in situ MHC-tetramer staining with immunohistochemistry to determine localization, phenotype, and quantity of antigen-specific T cells in tissues. This protocol is used to determine the spatial and phenotypic characteristics of antigen-specific CD8 T cells relative to other cell type and structures in tissues.

Tags

MHC-tetramer Staining Quantitative Analysis CD8 T Cells Antigen-specific Tissues Location Abundance Phenotype Spatial Distribution Procedure Fresh Tissue Scalpel Rhesus Macaque Spleen Tissue Agarose Embedding Microtome Thickness Setting Razor Blade
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