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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
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JoVE Journal Chemie
Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2′-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

DOI:
10.3791/56189-v

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11:37 min

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July 28, 2017

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,
1Department of Chemistry,University of Colorado Denver

Kapitel

  • 00:05Titel
  • 01:07Solid-phase Synthesis of RNA Oligonucleotides
  • 03:25Deprotection and Purification of RNA Oligonucleotides
  • 07:12Characterization via MALDI-TOF and RNA Structure Analysis vi CD
  • 09:24Results: Characterization of RNA Oligomers Containing 2′-Othiophenylmethyl Modification
  • 10:25Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This article provides a detailed procedure on the solid-phase synthesis, purification, and characterization of dodecamers of RNA modified at the C2'-O-position. UV-vis and circular dichroism photometric analyses are used to quantify and characterize structural aspects, i.e., single-strands or double-strands.

Tags

Solid-phase SynthesisOligomersRNA2′-O-thiophenylmethyl ModificationCircular DichroismPhosphoramidite ChemistryOligonucleotidesTherapeutic ApplicationsPeptide Synthesizer

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