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Developmental Biology

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Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing
 

Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing

Article DOI: 10.3791/56200-v 08:39 min September 1st, 2017
September 1st, 2017

Kapitel

Summary

The goal of this protocol is to alter the penetrance of lethal skeletal mutant phenotypes in zebrafish by selective breeding. Lethal mutants cannot be grown to adulthood and bred themselves, therefore this protocol describes a method for tracking and selecting penetrance through multiple generations by progeny testing.

Tags

Keywords: Zebrafish Lethal Skeletal Mutant Penetrance Progeny Testing Selective Breeding Recessive Lethal KASP Genotyping Genomic DNA PCR
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