Imaging Neural Activity in the Primary Somatosensory Cortex Using <em>Thy1</em>-GCaMP6s Transgenic Mice

Xiaojing Lin; Tingbao Zhao; Wenhui Xiong; Shaonan Wen; Xiaoming Jin; Xiao-Ming Xu

doi:10.3791/56297

Journal

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Neurowissenschaften

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Neuronalen Aktivität im primären somatosensorischen Cortex mit Thy1Imaging-GCaMP6s transgene Mäuse

Imaging Neural Activity in the Primary Somatosensory Cortex Using <em>Thy1</em>-GCaMP6s Transgenic Mice

JoVE Journal
Neurowissenschaften

This content is Free Access.

JoVE Journal Neurowissenschaften

Imaging Neural Activity in the Primary Somatosensory Cortex Using Thy1-GCaMP6s Transgenic Mice

Neuronalen Aktivität im primären somatosensorischen Cortex mit Thy1Imaging-GCaMP6s transgene Mäuse

Please note that all translations are automatically generated. Click here for the English version.

11,039 Views

•

07:04 min

•

January 07, 2019

DOI:

10.3791/56297-v

DOI:

10.3791/56297-v

•

07:04 min

January 07, 2019

•

10982 Views

Xiaojing Lin^1,2, Tingbao Zhao³, Wenhui Xiong¹, Shaonan Wen⁴, Xiaoming Jin¹, Xiao-Ming Xu¹

¹Spinal Cord and Brain Injury Research Group, Stark Neurosciences Research Institute, Department of Neurological Surgery and Goodman and Campbell Brain and Spine, Department of Anatomy and Cell Biology,Indiana University School of Medicine ²Department of Spinal Cord Injury and Repair, Trauma and Orthopedics Institute of Chinese PLA,General Hospital of Jinan Military Region ³Department of Orthopedics, Shandong Cancer Hospital,Shandong University ⁴Department of Neurobiology, Institute of Basic Medical Sciences,Academy Military Medical Sciences

Summary

Wir beschreiben ein experimentelles Verfahren zur Messung der neuronalen Aktivität durch dual optische Fenster über bilaterale primären somatosensorischen Corticies (S1) in Thy1-GCaMP6s transgene Mäuse mit 2-Photonen (2P) Mikroskopie in Vivo.

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Neuronalen Aktivität im primären somatosensorischen Cortex mit Thy1Imaging-GCaMP6s transgene Mäuse

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