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Frazionamento subcellulare per l'attivazione di ERK previo trattamento di Peptide derivato mitocondriale

Article DOI: 10.3791/56496-v • 07:55 min • September 25th, 2017
September 25th, 2017 •
1, 1, 1, 1
1Leonard Davis School of Gerontology, University of Southern California

Kapitel

Summary
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Questo protocollo descrive come stimolare le cellule con peptidi derivati mitocondriale e valutare la cascata di segnalazione e localizzazione di fosfo-proteine.

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Biologia cellulare numero 127 peptidi derivati Mitochondrial (MDPs) frazionamento di mitocondri ERK fosforilazione trattamento del peptide cytosol e frazionamento di nucleo
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