Synthese und Bewertung von Ruthenium-basierte mitochondrialen Kalzium-Aufnahme-Hemmer
7,776 Views
•
07:12 min
•
October 26, 2017
DOI:
Transkript
Automatically generated
Summary
Automatically generated
Ein Protokoll für die Synthese, Aufreinigung und Charakterisierung von einer Ruthenium-basierte Inhibitor der mitochondrialen Calciumaufnahme wird vorgestellt. Ein Verfahren zur Bewertung ihrer Wirksamkeit in permeabilized Säugerzellen wird demonstriert.
Verwandte Videos
10:51
The Synthesis, Characterization and Reactivity of a Series of Ruthenium N-triphosPh Complexes
Verwandte Videos
12070 Views
08:29
Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
Verwandte Videos
13722 Views
09:00
An In Vitro Enzymatic Assay to Measure Transcription Inhibition by Gallium(III) and H3 5,10,15-tris(pentafluorophenyl)corroles
Verwandte Videos
11494 Views
08:46
Dose Uptake of Platinum- and Ruthenium-based Compound Exposure in Zebrafish by Inductively Coupled Plasma Mass Spectrometry with Broader Applications
Verwandte Videos
2293 Views
08:03
Unveiling Xenobiotic Transport and Effects in Isolated Mitochondria: Insights from Respirometric and Enzymatic Assays
Verwandte Videos
461 Views
05:53
Mitochondrial Ca2+ Retention Capacity Assay and Ca2+-triggered Mitochondrial Swelling Assay
Verwandte Videos
11111 Views
13:42
Multi-parameter Measurement of the Permeability Transition Pore Opening in Isolated Mouse Heart Mitochondria
Verwandte Videos
21416 Views
08:43
Simultaneous Measurement of Mitochondrial Calcium and Mitochondrial Membrane Potential in Live Cells by Fluorescent Microscopy
Verwandte Videos
18837 Views
07:12
Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor
Verwandte Videos
7.8K Views
02:01
Mitochondrial Calcium Uptake Assay: A Plate Reader-Based Method for Measuring Calcium Uptake in Isolated Mitochondria
Verwandte Videos
443 Views
Read Article
Cite this Article
Nathan, S. R., Wilson, J. J. Synthesis and Evaluation of a Ruthenium-based Mitochondrial Calcium Uptake Inhibitor. J. Vis. Exp. (128), e56527, doi:10.3791/56527 (2017).
Copy