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En kinetisk fluorescens-baserad certifikatutfärdare2 + mobilisering analysen identifiera G-proteinkopplade receptoragonister och antagonister Allosteriska modulatorer

Article DOI: 10.3791/56780-v • 07:41 min • February 20th, 2018
February 20th, 2018 •
1, 1, 1, 1, 1
1Laboratory of Virology and Chemotherapy, Department of Microbiology and Immunology, Rega Institute for Medical Research, KU Leuven

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Beskrivna cellulära analysen är utformad för identifiering av CXC chemokine receptor 4 (CXCR4)-interagerande agenter som hämmar eller stimulera, antingen konkurrenskraftigt eller allosterically, intracellulära Ca2 + frisättning initierats av CXCR4 aktivering.

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Medicin fråga 132 G-proteinkopplade receptorer chemokine CXC chemokine receptor 4 fluorescens cell-baserad analys Ca2 + mobilisering agonist antagonist läkemedelsutveckling alloster modulator små molekyler
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