Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / 用 5-Bromouridine 标记和免疫沉淀 RNA…
JoVE Journal
Genetics

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

JoVE Journal Genetics
用 5-Bromouridine 标记和免疫沉淀 RNA 合成的研究
A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
Journal (Genetics)
A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
Journal (Genetics)
Targeted Next-generation Sequencing and Bioinformatics Pipeline to Evaluate Genetic Determinants of Constitutional Disease
 
Click here for the English version

用 5-Bromouridine 标记和免疫沉淀 RNA 合成的研究

Article DOI: 10.3791/57056-v • 09:59 min • May 3rd, 2018
May 3rd, 2018 •
1, 1, 2, 2, 1
1DANDRITE - Danish Research Institute of Translational Neuroscience, Dept. of Biomedicine, Aarhus University, 2Dept. of Molecular Biology and Genetics, Aarhus University

Kapitel

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

该方法可用于测量 RNA 的合成。5-Bromouridine 被添加到细胞中, 并纳入合成 RNA。rna 的合成是通过标记后立即提取 rna 来测量的, 其次是标记 rna 的 5 Bromouridine 靶向免疫沉淀, 并通过反向转录和定量聚合酶链反应进行分析。

Tags

遗传学 问题 135 RNA 合成 5-Bromouridine 标签 免疫沉淀 苯酚-氯仿萃取 反向转录 定量聚合酶链反应
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter