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Journal / Cancer Research / 正常结肠和结直肠癌患者内皮细胞的分离--一种改进的协议…
JoVE Journal
Cancer Research

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JoVE Journal Cancer Research
正常结肠和结直肠癌患者内皮细胞的分离--一种改进的协议
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正常结肠和结直肠癌患者内皮细胞的分离--一种改进的协议

Article DOI: 10.3791/57400-v • 10:54 min • April 4th, 2018
April 4th, 2018 •
1, 1, 1, 2, 3, 4, 4, 2, *2, *1
1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen, Friedrich-Alexander University of Erlangen-Nuremberg
* These authors contributed equally

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Summary
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肿瘤内皮细胞是肿瘤微环境和病程的重要决定因素。本文介绍了一种从人大肠癌和正常结肠分离纯化和存活的内皮细胞的协议, 用于药物检测和病机研究。

Tags

癌症研究 问题 134 内皮细胞 血管 大肠癌 肿瘤 正常结肠 CD31 VE 钙黏蛋白 CD105 血管内皮素分类 组织离解 肿瘤微环境 成纤维细胞
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