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Immunology and Infection

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Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Article DOI: 10.3791/57463-v • 06:52 min • May 21st, 2018
May 21st, 2018 •
1, 1
1Department of Laboratory Medicine, University of Washington

Kapitel

Summary
Automatic Translation

We describe the detection of NLRP3 inflammasome activation on cellular basis using fluorescence microscopy and staining for active caspase-1 and the adaptor, ASC. A lactate dehydrogenase release assay is presented to detect pyroptotic lysis on a population basis. These techniques can be adapted to study many aspects of inflammasome biology.

Tags

Inflammasome Activation Pyroptotic Cell Death Fluorescence Microscopy Lactate Dehydrogenase Release Assay Caspase-1 Activation Inflammasome Pathways Bone Marrow-derived Macrophages Medium Replacement Nigericin Glycine FAM-YVAD-FMK PBS Wash Paraformaldehyde Fixation Fluorescence Nuclear Dye Labeling
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