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Journal / Biochemistry / The Determination of Protease Specificity in…
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Biochemistry

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The Determination of Protease Specificity in Mouse Tissue Extracts by MALDI-TOF Mass Spectrometry: Manipulating PH to Cause Specificity Changes

Article DOI: 10.3791/57469-v • 09:47 min • May 25th, 2018
May 25th, 2018 •
1, 2, 1
1Department of Microbiology and Molecular Cell Biology, Nihon Pharmaceutical University, 2Department of Clinical Pharmaceutics, Nihon Pharmaceutical University

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Summary
Automatic Translation

Here, we present a protocol to determine protease specificity in crude mouse tissue extracts using MALDI-TOF spectrometry.

Tags

Protease Specificity MALDI-TOF Mass Spectrometry Mouse Tissue Extracts PH Manipulation Crude Extracts Lung Tissue Samples Homogenize Centrifuge Protein Concentration Digestion Buffers TPCK Trypsin Staphylococcus Au
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