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Journal / Biochemistry / Toeprinting Analysis of Translation Initiation…
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Biochemistry

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JoVE Journal Biochemistry
Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs
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Toeprinting Analysis of Translation Initiation Complex Formation on Mammalian mRNAs

Article DOI: 10.3791/57519-v • 10:37 min • May 10th, 2018
May 10th, 2018 •
1, 1,2,3
1Department of Chemistry and Biochemistry, Alberta RNA Research and Training Institute, University of Lethbridge, 2Department of Neuroscience and the Canadian Centre for Behavioral Neuroscience (CCBN), University of Lethbridge, 3Arnie Charbonneau Cancer Institute, University of Calgary

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Summary
Automatic Translation

Toeprinting aims to measure the ability of in vitro transcribed RNA to form translation initiation complexes with ribosomes under a variety of conditions. This protocol describes a method for toeprinting mammalian RNA and can be used to study both cap-dependent and IRES-driven translation.

Tags

Toeprinting Analysis Translation Initiation Complex Formation Mammalian MRNAs MRNA Translation IRES-mediated Translation Initiation Transcription Reactions RNA Purification Acid Phenol Extraction RNA Precipitation Sodium Acetate
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