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Optimized Scratch Assay for In Vitro Testing of Cell Migration with an Automated Optical Camera

Article DOI: 10.3791/57691-v • 05:27 min • August 8th, 2018
August 8th, 2018 •
1, 1
1Department of Science and Environment, Roskilde University

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Summary
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Here we describe a procedure to test the cell migration in vitro with an automated optical camera microscope. The scratch assay has been widely used where the closure of the scratch is followed for a set period. The optical microscope enables dependable and cheap detection of cell migration.

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Optimized Scratch Assay In Vitro Testing Cell Migration Automated Optical Camera Chronic Wounds Cancer Therapy HaCaT Cells Trypsin Incubation Enzymatic Reaction FBS Concentration Tissue Culture Plate Cell Culture Incubator Confluency Supernatant Replacement Mitomycin C Incubation Wash
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