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A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Article DOI: 10.3791/57831-v • 09:34 min • October 25th, 2018
October 25th, 2018 •
1, 1, 1, 1
1Division of Molecular Hematology, Lund Stem Cell Center, Lund University

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Summary
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Bulk gene expression measurements cloud individual cell differences in heterogeneous cell populations. Here, we describe a protocol for how single-cell gene expression analysis and index sorting by Florescence Activated Cell Sorting (FACS) can be combined to delineate heterogeneity and immunophenotypically characterize molecularly distinct cell populations.

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Combinatorial Single-cell Approach Molecular Heterogeneity Immunophenotypic Heterogeneity Human Stem Cells Progenitor Populations Stem Cell Heterogeneity Cancer Cells Therapy Isolation Single-cell Gene Expression Analysis Vaccinosis Downstream Characterization Gene Priming Lineage Potential Lysis Buffer RNA/DNA-free Bench NP-40 DNTP DTT RNAse Inhibitor PCR Plate Adhesive Film FACS Machine Target Population Cell Sorting Stain Buffer
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