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Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice
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JoVE Journal Biologie
Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice

Nasal Potential Difference to Quantify Trans-epithelial Ion Transport in Mice

DOI:
10.3791/57934-v

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08:55 min

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July 04, 2018

•

,
1Louvain Center for Toxicology and Applied Pharmacology (LTAP), Institut de Recherche Expérimentale et Clinique (IREC),Université Catholique de Louvain

Kapitel

  • 00:04Titel
  • 00:49Pre-test Assessment and Management
  • 01:59Assessment and Management During the Test
  • 06:55Results: Representative Nasal PD Tracings
  • 08:03Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here, we present a protocol to measure nasal potential difference in mice. The test quantifies the function of transmembrane ion transporters such as the cystic fibrosis transmembrane conductance regulator and the epithelial sodium channel. It is valuable to evaluate the efficacy of novel therapies for cystic fibrosis.

Tags

Nasal Potential DifferenceCystic FibrosisTransgenic MiceIon TransportDouble Lumen Nasal CatheterElectrolyte CreamVoltmeterPeristaltic PumpData Capture Software

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