Journal
/
Biochemie
/
Quantification of Hypopigmentation Activity In Vitro
JoVE Journal
Biochemie
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Biochemie
Quantification of Hypopigmentation Activity In Vitro

Quantification of Hypopigmentation Activity In Vitro

DOI:
10.3791/58185-v

•

06:08 min

•

March 06, 2019

•

, , , ,
1Department of Biotechnology, Graduate School of Life Sciences & Biotechnology, College of Life Sciences and Biotechnology,Korea University, 2JinWoo Bio Co. Ltd

Kapitel

  • 00:04Titel
  • 00:31Cellular Tyrosinase Activity and Melanin Content Measurement
  • 03:13Fontana-Masson Staining and Analysis
  • 05:02Results: Arbutin-Suppressed Melanogenesis in Melanocytes
  • 05:32Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We describe three experimental methods for evaluating the hypopigmentation activity of chemicals in vitro: quantification of 1) cellular tyrosinase activity and 2) melanin content, and 3) measurement of melanin by cellular melanin staining and image analysis.

Tags

HypopigmentationIn VitroTyrosinase ActivityB16-F10 MelanocytesMelanin ContentFontana-Masson StainingAnti-melanogenic ActivityCosmetic DevelopmentSkin Agents

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo

Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue

An In Vitro Assay to Detect tRNA-Isopentenyl Transferase Activity

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Detection of Phospholipase C Activity in the Brain Homogenate from the Honeybee

In Vitro Ubiquitination and Deubiquitination Assays of Nucleosomal Histones

A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

Synthesis of a Deuterated Standard for the Quantification of 2-Arachidonoylglycerol in Caenorhabditis elegans

Read Article