Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Generating Recombinant Avian Herpesvirus Vectors…
JoVE Journal
Immunology and Infection

Dieser Inhalt ist Open Access.

JoVE Journal Immunology and Infection
Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing
Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
Journal (Immunology and Infection)
Flow Cytometric Analysis of Lymphocyte Infiltration in Central Nervous System during Experimental Autoimmune Encephalomyelitis
Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
Journal (Immunology and Infection)
Cortical Actin Flow in T Cells Quantified by Spatio-temporal Image Correlation Spectroscopy of Structured Illumination Microscopy Data
 

Generating Recombinant Avian Herpesvirus Vectors with CRISPR/Cas9 Gene Editing

Article DOI: 10.3791/58193-v • 12:21 min • January 7th, 2019
January 7th, 2019 •
1,2, 1, 1, 1, 1, 1, 2, 1, 1
1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

Kapitel

Summary
Automatic Translation

Herpesvirus of turkeys (HVT) is widely used as a vector platform for the generation of recombinant vaccines against a number of avian diseases. This article describes a simple and rapid approach for the generation of recombinant HVT-vectored vaccines using an integrated NHEJ-CRISPR/Cas9 and Cre-Lox system.

Tags

Recombinant Avian Herpesvirus Vectors CRISPR/Cas9 Gene Editing Viral Antigens Recombinant Vaccines GFP Expression Cassette Cre-LoxP System Multivalent Recombinant Vaccines Chick Embryo Fibroblasts Cas9 Guide RNA Plasmids Donor Plasmid Transfection Reagent HVT Virus Stock Plaque Forming Units
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter