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Measuring Hypopharyngeal Gland Acinus Size in Honey Bee (Apis mellifera) Workers

Article DOI: 10.3791/58261-v • 05:11 min • September 14th, 2018
September 14th, 2018 •
1, 1
1USDA-ARS, Carl Hayden Bee Research Center

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Summary
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Hypopharyngeal gland acinus size is a robust measure of nurse honey bee nutrition. Here, we provide a detailed protocol for dissecting, staining, imaging, and measuring nurse bee hypopharyngeal gland acini.

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Hypopharyngeal Gland Acinus Size Honey Bee Apis Mellifera Nutrition Poor Nutrition Growth And Development Technique Stain Visualize Measure Locate Dissect Visual Guidance Procedure Melted Cool Setting Wax Glass Petri Dish Giemsa Stain Saline Microscope Slide Nurse Worker Bee Micro Spring Scissors Forceps Dissecting Microscope Wax Carving Pen Embed Head Front-side-up Wax Dissection Plate Pins Eyes Mouth Secure Head To Plate Sharp Razor Blade Incision Between Eyes And Mandibles
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