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Harvest of Endothelial Cells from the Balloon Tips of Swan-Ganz Catheters after Right Heart Catheterization
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Harvest of Endothelial Cells from the Balloon Tips of Swan-Ganz Catheters after Right Heart Catheterization

Article DOI: 10.3791/58353-v • 07:45 min • January 23rd, 2019
January 23rd, 2019 •
1, 1, 1, 1, 1
1Cardiovascular Institute, Allegheny Health Network

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Summary
Automatic Translation

This protocol describes the removal and purification of pulmonary artery endothelial cells (PAECs) that adhere to the balloon tips of Swan-Ganz catheters while they are wedged in the pulmonary artery during right heart catheterization and remain adherent to the deflated balloon following removal from the patient's body.

Tags

Harvest Endothelial Cells Balloon Tips Swan-Ganz Catheters Right Heart Catheterization Molecular Path Intimal Proliferation Pulmonary Hypertension Cellular Biopsy Risk To The Patient Clinical Coordinator Laboratory Manager Centrifuge Tube Sterile Saline Forceps Endothelial Cell-growth Medium Wet Ice Overnight Shipping
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