Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biochemistry

Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K
 

Use of Electron Paramagnetic Resonance in Biological Samples at Ambient Temperature and 77 K

Article DOI: 10.3791/58461-v 06:45 min January 11th, 2019
January 11th, 2019

Kapitel

Summary

Electron paramagnetic resonance (EPR) spectroscopy is an unambiguous method to measure free radicals. The use of selective spin probes allows for detection of free radicals in different cellular compartments. We present a practical, efficient method to collect biological samples that facilitate treating, storing, and transferring samples for EPR measurements.

Tags

Keywords: Electron Paramagnetic Resonance EPR Spectroscopy Redox Biology Reactive Oxygen Species Spin Probes Superoxide Detection 264.7 Cells Krebs-Henseleit Buffer DTPA Superoxide Dismutase CMH PMA Room Temperature 77 K
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter