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Immunology and Infection

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JoVE Journal Immunology and Infection
Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model
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Journal (Immunology and Infection)
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Journal (Immunology and Infection)
Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions
 

Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model

Article DOI: 10.3791/58768-v • 06:15 min • December 20th, 2018
December 20th, 2018 •
1, 1,2, 1,3, 2,4
1Institute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, 2Department of Internal Medicine, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, 3Department of Biochemistry and Molecular Biology, College of Medicine, National Cheng Kung University, 4Department of Microbiology and Immunology, College of Medicine, National Cheng Kung University

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Summary
Automatic Translation

Here, we introduce three different experiments to study Aeromonas infection in C. elegans. Using these convenient methods, it is easy to evaluate the toxicity among and within Aeromonas species.

Tags

Virulence Pathogenesis Aeromonas Infection Caenorhabditis Elegans Model Host-pathogen Interaction Bacterial Infection Toxicity Evaluation Pathogenesis Understanding Worm Lysis Egg Centrifugation M9 Medium L1 Worms Concentration E.coli OP50 LB Broth ENGM Plate Incubation
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