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Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture
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JoVE Journal Biotechnik
Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture

Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture

DOI:
10.3791/58791-v

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06:01 min

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February 02, 2019

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, , , ,
1Cell & Molecular Biology Program,University of Arkansas, 2Department of Biomedical Engineering,University of Arkansas, 3Ralph E. Martin Department of Biomedical Engineering,University of Arkansas

Kapitel

  • 00:04Titel
  • 00:34Hollow Fiber Membrane (HFM) Cell Seeding and Extracellular Matrix (ECM) Extraction
  • 03:06Extracellular Matrix Decellularization
  • 04:25Results: Representative ECM Implant Assembly
  • 05:21Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

The goal of this protocol is production of whole extracellular matrix fibers targeted for wound repair which are suitable for preclinical evaluation as part of a regenerative scaffold implant. These fibers are produced by the culture of fibroblasts on hollow fiber membranes and extracted by dissolution of the membranes.

Tags

Extracellular MatrixSacrificial Hollow Fiber MembraneCell CultureFibroblastsWound RepairBiomaterialTextile FabricationN-Methyl-2-pyrrolidoneSilicone Rubber Mold

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