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JoVE Journal
Bioengineering

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Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture
 

Production of Extracellular Matrix Fibers via Sacrificial Hollow Fiber Membrane Cell Culture

Article doi: 10.3791/58791
February 2nd, 2019

Summary February 2nd, 2019

The goal of this protocol is production of whole extracellular matrix fibers targeted for wound repair which are suitable for preclinical evaluation as part of a regenerative scaffold implant. These fibers are produced by the culture of fibroblasts on hollow fiber membranes and extracted by dissolution of the membranes.

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