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Journal / Neuroscience / Primary Cell Culture of Purified GABAergic or…
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Neuroscience

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JoVE Journal Neuroscience
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Primary Cell Culture of Purified GABAergic or Glutamatergic Neurons Established through Fluorescence-activated Cell Sorting

Article DOI: 10.3791/58974-v • 10:57 min • June 6th, 2019
June 6th, 2019 •
1, 1, 2, 3, 1
1Institut für Integrative Neuroanatomie, Charité, Universitätsmedizin Berlin, 2Department for Cytometry and Cellsorting, German Rheumatism Research Center, Leibniz Institute, 3Departments of Genetic and Behavioral Neuroscience, Graduate School of Medicine, Gunma University

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Summary
Automatic Translation

This protocol describes a cell sorting based method for the purification and culture of fluorescent GABAergic or glutamatergic neurons from the neocortex and hippocampus of postnatal mice or rats.

Tags

Primary Cell Culture Purified Neurons GABAergic Neurons Glutamatergic Neurons Fluorescence-activated Cell Sorting Electrophysiological Analysis Morphological Analysis Survival Analysis Neuronal Physiology Synapse Formation Neuronal Networks Cortical Glutamatergic Principal Cells GABAergic Interneurons Fluorescent Proteins Glass Coverslips PLL Solution Filter Sterilize Solution
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