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Cancer Research

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JoVE Journal Cancer Research
Langsgående morfologiske og fysiologiske overvågning af tre-dimensionelle Tumor Spheroids bruge optisk kohærens tomografi
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Langsgående morfologiske og fysiologiske overvågning af tre-dimensionelle Tumor Spheroids bruge optisk kohærens tomografi

Article DOI: 10.3791/59020-v • 08:50 min • February 9th, 2019
February 9th, 2019 •
1, 1, 1, 1, 5, 2, 3, 1, 4, 4, 4, 2,5, 4, 1,5,6
1Department of Electrical and Computer Engineering, Lehigh University, 2Department of Mechanical Engineering, Lehigh University, 3Department of Biomedical Engineering, Southern University of Science and Technology, 4Department of Technology R&D, Nexcelom Bioscience LLC, 5Department of Bioengineering, Lehigh University, 6Center for Photonics and Nanoelectronics, Lehigh University

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Optisk kohærens tomografi (OCT), en tre-dimensionel tænkelig teknologi, blev brugt til at overvåge og karakterisere vækst kinetik af flercellede tumor spheroids. Præcise volumetriske kvantificering af tumor spheroids ved hjælp af en voxel tælle tilgang og etiket-fri dødt væv påvisning i spheroids baseret på iboende optiske svækkelseskarakteristika kontrast, blev demonstreret.

Tags

Kræftforskning spørgsmålet 144 optisk kohærens tomografi OLT cellulære spheroids kulturperler tumorceller high throughput screening-assays tredimensional billeddannelse optiske billeddannelse antitumor stof screening assays
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