Waiting
Login-Verarbeitung ...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biochemistry

Dieser Inhalt ist Open Access.

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51
 

Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51

Article DOI: 10.3791/59073-v 06:24 min February 13th, 2019
February 13th, 2019

Kapitel

Summary

Fluorescence resonance energy transfer-based real-time observation systems of the DNA strand exchange reaction mediated by Rad51 were developed. Using the protocols presented here, we are able to detect the formation of reaction intermediates and their conversion into products, while also analyzing the enzymatic kinetics of the reaction.

Tags

Keywords: DNA Strand Exchange Rad51 Real-time Observation Recombination Proteins DNA Pairing Assay Fluorescence Spectroscopy FRET
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter