Echtzeit-Beobachtung der DNA-Strang Exchange Reaktion vermittelt durch Rad51
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February 13, 2019
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Fluoreszenz Resonanz Energie Transfer-basierte Echtzeit-Beobachtung, dass Systeme der DNA Strang Austausch Reaktion vermittelt durch Rad51 wurden entwickelt. Mithilfe der hier vorgestellten Protokolle, sind wir in der Lage, die Bildung von Zwischenprodukten Reaktion und ihre Umsetzung in Produkte, bei der Analyse auch die enzymatischen Kinetics der Reaktion zu erkennen.
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Ito, K., Argunhan, B., Tsubouchi, H., Iwasaki, H. Real-time Observation of the DNA Strand Exchange Reaction Mediated by Rad51. J. Vis. Exp. (144), e59073, doi:10.3791/59073 (2019).
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