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JoVE Journal
Biochemistry

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Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription
 

Application of Biolayer Interferometry (BLI) for Studying Protein-Protein Interactions in Transcription

Article DOI: 10.3791/59687-v 07:18 min July 26th, 2019
July 26th, 2019

Kapitel

Summary

Interactions of transcription factors (TFs) with the RNA polymerase are usually studied using pulldown assays. We apply a Biolayer Interferometry (BLI) technology to characterize the interaction of GrgA with the chlamydial RNA polymerase. Compared to pulldown assays, BLI detects real-time association and dissociation, offers higher sensitivity, and is highly quantitative.

Tags

Keywords: Biolayer Interferometry BLI Protein-protein Interactions Transcription Kinetic Parameters Biosensor Nickel NTA Real-time Association And Dissociation Quantitative Analysis Por Dialysis
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