JoVE Journal
Biochemistry
Biochemistry
Ein Abonnement für JoVE ist erforderlich, um diesen Inhalt ansehen zu können.
Kapitel
Summary
Interactions of transcription factors (TFs) with the RNA polymerase are usually studied using pulldown assays. We apply a Biolayer Interferometry (BLI) technology to characterize the interaction of GrgA with the chlamydial RNA polymerase. Compared to pulldown assays, BLI detects real-time association and dissociation, offers higher sensitivity, and is highly quantitative.