Assessing the Autonomic and Behavioral Effects of Passive Motion in Rats using Elevator Vertical Motion and Ferris-Wheel Rotation

Francis A. M. Manno; Leilei Pan; Yuqi Mao; Yang Su; Sinai  H. C. Manno; Shuk  Han Cheng; Condon Lau; Yiling Cai

doi:10.3791/59837

Bewertung der autonomen und Verhaltenseffekte passiver Bewegungen bei Ratten mit vertikaler Aufzu...

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Behavior
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JoVE Journal Behavior

Assessing the Autonomic and Behavioral Effects of Passive Motion in Rats using Elevator Vertical Motion and Ferris-Wheel Rotation

Bewertung der autonomen und Verhaltenseffekte passiver Bewegungen bei Ratten mit vertikaler Aufzugsbewegung und Riesenradrotation

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06:18 min

February 7, 2020

DOI: 10.3791/59837-v

Francis A. M. Manno^1,2, Leilei Pan³, Yuqi Mao³, Yang Su³, Sinai H. C. Manno^2,4,5, Shuk Han Cheng^4,5,6, Condon Lau², Yiling Cai³

¹School of Biomedical Engineering, Faculty of Engineering,University of Sydney, ²Department of Physics,City University of Hong Kong, ³Department of Nautical Injury Prevention, Faculty of Navy Medicine,Second Military Medical University, ⁴State Key Laboratory of Marine Pollution (SKLMP),City University of Hong Kong, ⁵Department of Biomedical Sciences, College of Veterinary Medicine and Life Sciences,City University of Hong Kong, ⁶Department of Materials Science and Engineering,City University of Hong Kong

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Overview

This article presents protocols to assess the autonomic and behavioral effects of passive motion in rodents. Techniques such as elevator vertical motion and Ferris-wheel rotation are utilized to evaluate the integrity of the autonomic nervous system.

Key Study Components

Area of Science

Neuroscience
Behavioral Analysis
Autonomic Nervous System

Background

Protocols are essential for assessing autonomic nervous system functionality.
Vestibular system disorders can be indicated by deficits in motor coordination.
Behavioral techniques may be influenced by experimenter variability.
Consistency in handling rodents is crucial for reliable results.

Purpose of Study

To establish the integrity of the autonomic system.
To assess the functionality of the autonomic nervous system.
To identify potential vestibular system disorders.

Methods Used

Elevator vertical motion analysis with rodents.
Ferris-wheel rotation protocols.
Use of a controlled environment for testing.
Standardized handling of subjects throughout the experiment.

Main Results

Protocols effectively reveal the integrity of autonomic functioning.
Consistent handling reduces variability in behavioral assessments.
Identified techniques can signal autonomic system damage.
Behavioral outcomes correlate with vestibular system health.

Conclusions

Protocols are reliable for assessing autonomic and behavioral effects.
Researcher consistency is vital for experimental integrity.
Techniques can aid in diagnosing vestibular and autonomic disorders.

Frequently Asked Questions

What is the significance of assessing the autonomic nervous system?

Assessing the autonomic nervous system is crucial for understanding its functionality and identifying potential disorders.

How do the protocols help in behavioral analysis?

The protocols provide standardized methods to minimize variability and enhance the reliability of behavioral assessments.

Why is researcher consistency important?

Consistency in handling subjects ensures that results are not influenced by different handling techniques, leading to more accurate data.

What types of rodents are used in the study?

The study typically uses two-month-old rats weighing between 200 to 250 grams.

What are the implications of vestibular system disorders?

Vestibular system disorders can indicate underlying issues with the autonomic nervous system and affect motor coordination.

How are the protocols implemented in practice?

Protocols involve placing rodents in a controlled environment and subjecting them to specific motion analyses.

Protokolle werden vorgestellt, um die autonomen und verhaltensorientierten Auswirkungen passiver Bewegungen bei Nagetieren mittels vertikaler Aufzugsbewegung und Riesenraddrehung zu bewerten.

Diese Protokolle sind wichtig für die Integrität und das normale Funktionieren des autonomen Systems, um die Funktionalität des autonomen Nervensystems zu bewerten und vestibuläre Systemstörungen aufzudecken. Ein vestibuläres Funktions- oder Motorkoordinationsdefizit kann Schäden am autonomen System signalisieren. Diese Techniken können die Integrität der autonomen Funktion offenbaren.

Verhaltenstechniken können anfällig für Vom Experimentator eingeführte Variabilitäten sein. Wir empfehlen dem gleichen Forscher, die Techniken während des gesamten Experiments durchzuführen und jedes Nagetier gleich zu behandeln. Für die vertikale Aufzugsbewegungsanalyse bis zu vier zwei Monate alte 200 bis 250 Gramm Ratten in eine 22,5 mal 26 mal 20 Zentimeter große Plexiglasbox legen und die Box sicher gestalten.

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