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Journal / Neuroscience / 抗体喂养方法研究分离原发性希马培养物中的谷氨酸受体贩运…
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Neuroscience

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JoVE Journal Neuroscience
抗体喂养方法研究分离原发性希马培养物中的谷氨酸受体贩运
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抗体喂养方法研究分离原发性希马培养物中的谷氨酸受体贩运

Article DOI: 10.3791/59982-v • 08:13 min • August 2nd, 2019
August 2nd, 2019 •
1, 1, 1,2, 1
1Department of Pharmacology, Feinberg School of Medicine, Northwestern University, 2Department of Cellular Neurophysiology, Institute of Physiology CAS

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本文提出了一种研究分离原发海马培养物的谷氨酸受体(GluR)贩运的方法。使用抗体喂养方法将内源性或过度表达的受体与药理学方法相结合,该方法允许通过调节来识别调节GluR表面表达的分子机制内部化或回收过程。

Tags

神经科学 问题 150 谷氨酸受体 表面表达 受体内化 受体回收 抗体喂养 转染 神经元
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